gpr61 tango plasmid dna Search Results


hibit gpr61 plasmid dna  (Addgene inc)
93
Addgene inc hibit gpr61 plasmid dna
RMSD of carbon alpha atoms from the three simulations of the <t>GPR61</t> WT ( A ) and GPR61 R236C ( B ).
Hibit Gpr61 Plasmid Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gpr61+tango+plasmid+dna/GPR61-Tango+(Plasmid+%2366366)/med_rxiv__2024__10__21__24315834-48-0-13
Average 93 stars, based on 1 article reviews
hibit gpr61 plasmid dna - by Bioz Stars, 2026-10
93/100 stars
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ht3a signal peptide plasmid dna  (Addgene inc)
93
Addgene inc ht3a signal peptide plasmid dna
RMSD of carbon alpha atoms from the three simulations of the <t>GPR61</t> WT ( A ) and GPR61 R236C ( B ).
Ht3a Signal Peptide Plasmid Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gpr61+tango+plasmid+dna/2xDHFR-myc+(Plasmid+%2320215)/pm40133016-79-29-17
Average 93 stars, based on 1 article reviews
ht3a signal peptide plasmid dna - by Bioz Stars, 2026-10
93/100 stars
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pcmv6  (OriGene)
91
OriGene pcmv6
RMSD of carbon alpha atoms from the three simulations of the <t>GPR61</t> WT ( A ) and GPR61 R236C ( B ).
Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gpr61+tango+plasmid+dna/pCMV6-AN-His-Myc+Mammalian+Expression+Vector/pm29226084-41-12-14
Average 91 stars, based on 1 article reviews
pcmv6 - by Bioz Stars, 2026-10
91/100 stars
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Image Search Results


RMSD of carbon alpha atoms from the three simulations of the GPR61 WT ( A ) and GPR61 R236C ( B ).

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: RMSD of carbon alpha atoms from the three simulations of the GPR61 WT ( A ) and GPR61 R236C ( B ).

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques:

The same procedure was used for GPR61, MC 4 R and GPR141.

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: The same procedure was used for GPR61, MC 4 R and GPR141.

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques:

A. Mutational landscape of GPR61 in normal population and then the same mutations in severe obesity patients. B. GPR61 mutations mapped onto a 2D model of GPR61.

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: A. Mutational landscape of GPR61 in normal population and then the same mutations in severe obesity patients. B. GPR61 mutations mapped onto a 2D model of GPR61.

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques:

PolyPhen-2 analysis of the 34 mutations in GPR61 to predict their impact on structure and function of the protein.

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: PolyPhen-2 analysis of the 34 mutations in GPR61 to predict their impact on structure and function of the protein.

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques:

NanoBiT-based measurement of cell surface expression of 34 mutants of GPR61. The red bars indicate statistically significant decrease and the green bars indicate statistically significant increase in NanoBiT-emmitted cell membrane-associated luminescence indicative of cell surface expression of HiBiT-tagged GPR61 constructs; at least p<0.05 and at least n=3 independent experiments.

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: NanoBiT-based measurement of cell surface expression of 34 mutants of GPR61. The red bars indicate statistically significant decrease and the green bars indicate statistically significant increase in NanoBiT-emmitted cell membrane-associated luminescence indicative of cell surface expression of HiBiT-tagged GPR61 constructs; at least p<0.05 and at least n=3 independent experiments.

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques: Expressing, Membrane, Construct

A. EPAC-derived FRET-based biosensor reveals that three mutations (the red bars) lead to the statistically significant reduction in cAMP production elicited by overexpression of a GPR61 in the absence of an agonist (increase in the FRET ratio reflects decrease in cAMP levels). B. The presence of overexpressed GPR61 R236C leads to a statistically-significant reduction in the bystander BRET ratio between Gαs-67-RlucII and rGFP-CAAX indicative of reduced G s translocation away from the cell membrane; at least p<0.05 and at least n=3 independent experiments.

Journal: medRxiv

Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

doi: 10.1101/2024.10.21.24315834

Figure Lengend Snippet: A. EPAC-derived FRET-based biosensor reveals that three mutations (the red bars) lead to the statistically significant reduction in cAMP production elicited by overexpression of a GPR61 in the absence of an agonist (increase in the FRET ratio reflects decrease in cAMP levels). B. The presence of overexpressed GPR61 R236C leads to a statistically-significant reduction in the bystander BRET ratio between Gαs-67-RlucII and rGFP-CAAX indicative of reduced G s translocation away from the cell membrane; at least p<0.05 and at least n=3 independent experiments.

Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

Techniques: Derivative Assay, Over Expression, Translocation Assay, Membrane